NIH Image Tutorial:
Measuring the Eye
- Open NIH Image. Use File>Open to open
the stack file you created in the previous
- In Analyze>Set Scale enter 4.200 pixels per mm.
- Set your parameters to measure in
Analyze>Options. Click on the Area
and Perimeter/Length boxes to leave them
- Open Analyze>Show Results. A box will
appear with the measurement options that you have chosen
in step 3, but no measurements. As you measure the area
of the eye for each image in your stack, that
measurement will appear in the Results box. This
way you will know if you have accidently measured an
area twice. If you have measured the image twice, use
Analyze>Delete Measuremet. Use
Analyze>Redo Measurement if you are not
satified with the measurement you took.
- Use the zoom tool to zoom into the eye on the first
image in the stack.
- Use the trace tool to select the eye by dragging
the cursor around the perimeter of the eye.
- Analyze>Measure the area you have chosen
in slice 1. Do this only once; if you do it more than
once you will get more than one entry in the resulting
data table. If you want to do it over, use
Analyze>Redo Measurement. Use
Analyze>Delete Measurement to remove the
extra measurement before preceding.
- Edit>Deselect before going on to the next
- Go to the next slice using Stacks>Next
- Repeat steps 7-10 on each of the slices in the
- Click on the Results Box to make it active then use
File>Save As to save your data. Click on the
Measurement button in the Save As pop-up
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